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Plos One : Orientation-specificity of Adaptation ; Isotropic Adapt...

By: Daniel Osorio

Description : Numerous studies have found that prolonged exposure to grating stimuli reduces sensitivity to subsequently presented gratings, most evidently when the orientations of the adapting and test patterns are similar. The rate of sensitivity loss varies with angular difference indicating both the presence and bandwidths of psychophysical ‘orientation channels’. Here we study the orientation dependency of contrast adaptation measured both monoptically and dichoptic...

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Plos One : Quantitation of Gene Expression in Formaldehyde-fixed a...

By: Yuntao Wu

Description : Fluorescence-activated cell sorting (FACS) is a sensitive and valuable technique to characterize cellular subpopulations and great advances have been made using this approach. Cells are often fixed with formaldehyde prior to the sorting process to preserve cell morphology and maintain the expression of surface molecules, as well as to ensure safety in the sorting of infected cells. It is widely recognized that formaldehyde fixation alters RNA and DNA struct...

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Plos One : the Coexistence of Asthma and Chronic Obstructive Pulmo...

By: Antony Bayer

Description : Asthma and COPD are common in the general population, and they coexist in a substantial proportion of subjects. The asthma-COPD overlap syndrome represents an important clinical phenotype that deserves more medical attention and further research

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Plos One : Ros Production is Essential for the Apoptotic Function ...

By: Daniel Sanchis

Description : In this study we demonstrate that accumulation of reactive oxygen species (ROS) is essential for E2F1 mediated apoptosis in ER-E2F1 PC12 pheochromocytoma, and SH-SY5Y and SK-N-JD neuroblastoma stable cell lines. In these cells, the ER-E2F1 fusion protein is expressed in the cytosol: the addition of 4-hydroxytamoxifen (OHT) induces its translocation to the nucleus and activation of E2F1target genes. Previously we demonstrated that, in ER-E2F1 PC12 cells, OHT...

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Plos One : Dna Methylation in the Malignant Transformation of Meni...

By: Jasti Rao

Description : Meningiomas are central nervous system tumors that originate from the meningeal coverings of the brain and spinal cord. Most meningiomas are pathologically benign or atypical, but 3–5% display malignant features. Despite previous studies on benign and atypical meningiomas, the key molecular pathways involved in malignant transformation remain to be determined, as does the extent of epigenetic alteration in malignant meningiomas. In this study, we explored t...

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Plos One : Sexually Dimorphic Genome-wide Binding of Retinoid X Re...

By: Wendong Huang

Description : Many hepatic functions including lipid metabolism, drug metabolism, and inflammatory responses are regulated in a sexspecific manner due to distinct patterns of hepatic gene expression between males and females. Regulation for the majority of these genes is under control of Nuclear Receptors (NRs). Retinoid X Receptor alpha (RXRa) is an obligate partner for multiple NRs and considered a master regulator of hepatic gene expression, yet the full extent of RXR...

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Plos One : Distinct Ubiquitin Binding Modes Exhibited by Sh3 Domai...

By: Laszlo Buday

Description : SH3 domains constitute a new type of ubiquitin-binding domains. We previously showed that the third SH3 domain (SH3-C) of CD2AP binds ubiquitin in an alternative orientation. We have determined the structure of the complex between first CD2AP SH3 domain and ubiquitin and performed a structural and mutational analysis to decipher the determinants of the SH3-C binding mode to ubiquitin. We found that the Phe-to-Tyr mutation in CD2AP and in the homologous CIN8...

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Plos One : Diversity and Composition of Demersal Fishes Along a De...

By: Brian R. MacKenzie

Description : This study provides the first characterization of diversity patterns for bait-attracted fish species on continental slopes in New Zealand and is an imperative primary step towards development of explanatory and predictive ecological models, as well as being fundamental for the implementation of efficient management and conservation strategies for fishery resources.

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Plos One : High Content Screening as High Quality Assay for Biolog...

By: Jörg D. Hoheisel

Description : A novel single step assay approach to screen a library of photdynamic therapy (PDT) compounds was developed. Utilizing high content analysis (HCA) technologies several robust cellular parameters were identified, which can be used to determine the phototoxic effects of porphyrin compounds which have been developed as potential anticancer agents directed against esophageal carcinoma. To demonstrate the proof of principle of this approach a small detailed stud...

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Plos One : Molecular Phenotyping of Immune Cells from Young Nod Mi...

By: Gualtiero Colombo

Description : Islet leukocytic infiltration (insulitis) is first obvious at around 4 weeks of age in the NOD mouse – a model for human type 1 diabetes (T1D). The molecular events that lead to insulitis and initiate autoimmune diabetes are poorly understood. Since TID is caused by numerous genes, we hypothesized that multiple molecular pathways are altered and interact to initiate this disease. We evaluated the molecular phenotype (mRNA and protein expression) and molecul...

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Plos One : Deletion of Integron-associated Gene Cassettes Impact o...

By: Laurence Van Melderen

Description : From this study, it was concluded that deletion of gene cassettes had a subtle effect on bacterial metabolism but altered host surface polysaccharide. Deletion (and most likely rearrangement and acquisition) of gene cassettes may provide the bacterium with a mechanism to alter its surface properties, thus impacting on phenotypes such as biofilm formation. Biofilm formation was shown to be altered in one of the deletion mutants used in this study. Reworking ...

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Plos One : Association of Stat4 Polymorphisms with Susceptibility ...

By: Silvia C. Sookoian

Description : This is the first study to show a positive association between a STAT4 polymorphism and type-1 autoimmune hepatitis, suggesting that autoimmune hepatitis shares a gene commonly associated with risk for other autoimmune diseases

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Plos One : Esr Study of Interfacial Hydration Layers of Polypeptid...

By: Danilo Roccatano

Description : There is considerable evidence for the essential role of surface water in protein function and structure. However, it is unclear to what extent the hydration water and protein are coupled and interact with each other. Here, we show by ESR experiments (cw, DEER, ESEEM, and ESE techniques) with spin-labeling and nanoconfinement techniques that the vitrified hydration layers can be evidently recognized in the ESR spectra, providing nanoscale understanding for ...

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Plos One : Prognostic Value of Hpv E6, Volume 8

By: Jason Mulvenna

Description : Pap test, and especially HPV DNA test, identify a large group of women who do not have any clinically relevant lesions, i.e., CIN2+ (Cervical Intraepithelial Neoplasia grade 2 or worse), but who are at greater risk of getting lesions in the future. The follow up of these women needs new biomarkers with prognostic value. The objective of this study is to evaluate the prognostic value of E6/E7 mRNA over-expression assay (PreTect HPV-Proofer, Norchip) for 5 HR...

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Plos One : Effects of Abortion Legalization in Nepal, 2001–2010, V...

By: Colette Kanellopoulos-langevin

Description : Over the study period, health care use and the population of reproductive aged women increased. Total fertility also declined by nearly half, despite relatively low contraceptive prevalence. Greater numbers of women likely obtained abortions and sought hospital care for complications following legalization, yet we observed a significant decline in the rate of serious abortion morbidity. The liberalization of abortion policy in Nepal has benefited women’s he...

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Plos One : Cloning, Expression and Characterization of a Gene from...

By: Jitesh Pratap

Description : A lumbrokinase gene encoding a blood-clot dissolving protein was cloned from earthworm (Eisenia fetida) by RT-PCR amplification. The gene designated as CST1 (GenBank No. AY840996) was sequence analyzed. The cDNA consists of 888 bp with an open reading frame of 729 bp, which encodes 242 amino acid residues. Multiple sequence alignments revealed that CST1 shares similarities and conserved amino acids with other reported lumbrokinases. The amino acid sequence ...

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Plos One : Plasma Nov, Volume 8

By: Jean-marc A. Lobaccaro

Description : Our results strongly suggest that in obese humans and mice plasma NOV levels positively correlated with NOV expression in adipose tissue, and support a possible contribution of NOV to obesity-related inflammation.

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Plos One : Species Richness-environment Relationships of European ...

By: Hans Henrik Bruun

Description : We study how species richness of arthropods relates to theories concerning net primary productivity, ambient energy, water-energy dynamics and spatial environmental heterogeneity. We use two datasets of arthropod richness with similar spatial extents (Scandinavia to Mediterranean), but contrasting spatial grain (local habitat and country). Samples of grounddwelling spiders, beetles, bugs and ants were collected from 32 paired habitats at 16 locations across...

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Plos One : Patterns of Deep-sea Genetic Connectivity in the New Ze...

By: David Mark Bailey

Description : Patterns of genetic connectivity are increasingly considered in the design of marine protected areas (MPAs) in both shallow and deep water. In the New Zealand Exclusive Economic Zone (EEZ), deep-sea communities at upper bathyal depths (,2000 m) are vulnerable to anthropogenic disturbance from fishing and potential mining operations. Currently, patterns of genetic connectivity among deep-sea populations throughout New Zealand’s EEZ are not well understood. U...

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Plos One : Contribution of Common Genetic Variants to Obesity and ...

By: Joseph Devaney

Description : Our findings replicate the association of 8 obesity-related SNPs with obesity risk in Mexican adults, and confirm the role of some of these SNPs in BMI in Mexican adults and children.

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