World Library  


 
  • Cover Image

Plos Biology : an Unbiased Cell Morphology–based Screen for New, B...

By: Tom Misteli

Description : We have implemented an unbiased cell morphology–based screen to identify small-molecule modulators of cellular processes using the Cytometrix (TM) automated imaging and analysis system. This assay format provides unbiased analysis of morphological effects induced by small molecules by capturing phenotypic readouts of most known classes of pharmacological agents and has the potential to read out pathways for which little is known. Four human-cancer cell line...

Read More
  • Cover Image

Plos Biology : Research Chimpanzees May Get a Break, Volume 10

By: Public Library of Science (Plos)
Read More
  • Cover Image

Plos Biology : Specialization Does Not Predict Individual Efficien...

By: Anna Dornhaus

Description : The ecological success of social insects is often attributed to an increase in efficiency achieved through division of labor between workers in a colony. Much research has therefore focused on the mechanism by which a division of labor is implemented, i.e., on how tasks are allocated to workers. However, the important assumption that specialists are indeed more efficient at their work than generalist individuals—the ‘‘Jack-of-all-trades is master of none’’ ...

Read More
  • Cover Image

Plos Biology : Repeating Patterns of Mimicry, Volume 4

By: Axel Meyer
Read More
  • Cover Image

Plos Biology : Linking Splicing to Pol II Transcription Stabilizes...

By: Marv Wickens

Description : RNA processing is carried out in close proximity to the site of transcription, suggesting a regulatory link between transcription and pre-mRNA splicing. Using an in vitro transcription/splicing assay, we demonstrate that an association of RNA polymerase II (Pol II) transcription and pre-mRNA splicing is required for efficient gene expression. Pol IIsynthesized RNAs containing functional splice sites are protected from nuclear degradation, presumably because...

Read More
  • Cover Image

Plos Biology : Sirt1 Regulates Hiv Transcription Via Tat Deacetyla...

By: Michael Emerman

Description : The human immunodeficiency virus (HIV) Tat protein is acetylated by the transcriptional coactivator p300, a necessary step in Tat-mediated transactivation. We report here that Tat is deacetylated by human sirtuin 1 (SIRT1), a nicotinamide adenine dinucleotide-dependent class III protein deacetylase in vitro and in vivo. Tat and SIRT1 coimmunoprecipitate and synergistically activate the HIV promoter. Conversely, knockdown of SIRT1 via small interfering RNAs ...

Read More
  • Cover Image

Plos Biology : Bridging the Science–policy Divide, Volume 2

By: Walter V. Reid
Read More
  • Cover Image

Plos Biology : Response to ‘‘the Reality of Pervasive Transcriptio...

By: Michael B. Eisen
Read More
  • Cover Image

Plos Biology : ‘‘deconstructing’’ Scientific Research ; a Practica...

By: Cheryl A. Kerfeld
Read More
  • Cover Image

Plos Biology : Dynamics and Design Principles of a Basic Regulator...

By: Chen-shan Chin

Description : The dynamic features of a genetic network’s response to environmental fluctuations represent essential functional specifications and thus may constrain the possible choices of network architecture and kinetic parameters. To explore the connection between dynamics and network design, we have analyzed a general regulatory architecture that is commonly found in many metabolic pathways. Such architecture is characterized by a dual control mechanism, with end pr...

Read More
  • Cover Image

Plos Biology : Molecular Insights Into Human Brain Evolution, Volume 3

By: Jane Bradbury
Read More
  • Cover Image

Plos Biology : Spatial Learning Depends on Both the Addition and R...

By: Edvard Moser

Description : The role of adult hippocampal neurogenesis in spatial learning remains a matter of debate. Here, we show that spatial learning modifies neurogenesis by inducing a cascade of events that resembles the selective stabilization process characterizing development. Learning promotes survival of relatively mature neurons, apoptosis of more immature cells, and finally, proliferation of neural precursors. These are three interrelated events mediating learning. Thus,...

Read More
  • Cover Image

Plos Biology : the Case for Open Preprints in Biology, Volume 11

By: Philippe Desjardins-proulx

Description : Public preprint servers allow authors to make manuscripts publicly available before, or in parallel to, submitting them to journals for traditional peer review. The rationale for preprint servers is fundamentally simple : to make the results of research available to the scientific community as soon as possible, instead of waiting until the peer-review process is fully completed. Sharing manuscripts using preprint servers has numerous advantages, including :...

Read More
  • Cover Image

Plos Biology : Persistent Camp-signals Triggered by Internalized G...

By: Mark Von Zastrow

Description : G-protein–coupled receptors (GPCRs) are generally thought to signal to second messengers like cyclic AMP (cAMP) from the cell surface and to become internalized upon repeated or prolonged stimulation. Once internalized, they are supposed to stop signaling to second messengers but may trigger nonclassical signals such as mitogen-activated protein kinase (MAPK) activation. Here, we show that a GPCR continues to stimulate cAMP production in a sustained manner ...

Read More
  • Cover Image

Plos Biology : Disease Ecology, Biodiversity, and the Latitudinal ...

By: Terri L. Gilbert

Description : While most of the world is thought to be on long-term economic growth paths, more than one-sixth of the world is roughly as poor today as their ancestors were hundreds of years ago. The majority of the extremely poor live in the tropics. The latitudinal gradient in income is highly suggestive of underlying biophysical drivers, of which disease conditions are an especially salient example. However, conclusions have been confounded by the simultaneous causali...

Read More
  • Cover Image

Plos Biology : Linking Biodiversity Conservation and Livelihoods i...

By: Kartik Shanker
Read More
  • Cover Image

Plos Biology : Linking Stochastic Fluctuations in Chromatin Struct...

By: Oliver J. Rando

Description : The number of mRNA and protein molecules expressed from a single gene molecule fluctuates over time. These fluctuations have been attributed, in part, to the random transitioning of promoters between transcriptionally active and inactive states, causing transcription to occur in bursts. However, the molecular basis of transcriptional bursting remains poorly understood. By electron microscopy of single PHO5 gene molecules from yeast, we show that the ‘‘activ...

Read More
  • Cover Image

Plos Biology : a Genetically Encoded Tag for Correlated Light and ...

By: J. Richard McIntosh

Description : Electron microscopy (EM) achieves the highest spatial resolution in protein localization, but specific protein EM labeling has lacked generally applicable genetically encoded tags for in situ visualization in cells and tissues. Here we introduce ‘‘miniSOG’’ (for mini Singlet Oxygen Generator), a fluorescent flavoprotein engineered from Arabidopsis phototropin 2. MiniSOG contains 106 amino acids, less than half the size of Green Fluorescent Protein. Illumina...

Read More
  • Cover Image

Plos Biology : Model Selection and the Molecular Clock, Volume 4

By: Oliver G. Pybus
Read More
  • Cover Image

Plos Biology : Preserving a Space for Science in an Age of Democra...

By: Harry Collins
Read More
 
1
|
2
|
3
Records: 1 - 20 of 45 - Pages: 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.