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Plos One : Structural Characterization of a Recombinant Fusion Pro...

By: David G. Norman

Description : Conbercept is a genetically engineered homodimeric protein for the treatment of wet age-related macular degeneration (wet AMD) that functions by blocking VEGF-family proteins. Its huge, highly variable architecture makes characterization and development of a functional assay difficult. In this study, the primary structure, number of disulfide linkages and glycosylation state of conbercept were characterized by high-performance liquid chromatography, mass sp...

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Plos One : Genetic Variation in the Human Brain Dopamine System In...

By: Veronique Sgambato-faure

Description : Dopamine is important to learning and plasticity. Dopaminergic drugs are the focus of many therapies targeting the motor system, where high inter-individual differences in response are common. The current study examined the hypothesis that genetic variation in the dopamine system is associated with significant differences in motor learning, brain plasticity, and the effects of the dopamine precursor L-Dopa. Skilled motor learning and motor cortex plasticity...

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Plos One : Deep Sequencing Reveals Complex Spurious Transcription ...

By: Thomas Preiss

Description : Transient plasmid transfection is a common approach in studies in cultured mammalian cells. To examine behavior of transfected plasmids, we analyzed their transcriptional landscape by deep sequencing. We have found that the entire plasmid sequence is transcribed at different levels. Spurious transcription may have undesirable effects as some plasmids, when co-transfected, inhibited expression of luciferase reporters in a dose-dependent manner. In one case, ...

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Plos One : Cell Wall Assembly and Intracellular Trafficking in Pla...

By: Meng-xiang Sun

Description : Plants are able to sense the magnitude and direction of gravity. This capacity is thought to reside in selected cell types within the plant body that are equipped with specialized organelles called statoliths. However, most plant cells do not possess statoliths, yet they respond to changes in gravitational acceleration. To understand the effect of gravity on the metabolism and cellular functioning of non-specialized plant cells, we investigated a rapidly gr...

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Plos One : Spak Deficiency Corrects Pseudohypoaldosteronism II Cau...

By: Jean-claude Dussaule

Description : Stimulation of the OSR1 (Oxidative stress-responsive kinase-1)/SPAK [STE20 (sterile 20)/SPS1-related proline/alanine-rich kinase]-NCC (Na+-Cl2 cotransporter) signaling cascade plays an important role in the WNK [With-No-Lysine (K)] kinase 4 D561A knock-in mouse model of pseudohypoaldosteronism type II (PHA II) characterized by salt-sensitive hypertension and hyperkalemia. The aim of this study was to investigate the respective roles of Osr1 and Spak in the ...

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Plos One : Cancer-associated Fibroblasts from Hepatocellular Carci...

By: Song Guo Zheng

Description : Cancer-associated fibroblasts (CAFs) are reported to support tumorigenesis by stimulating angiogenesis, cancer cell proliferation, and invasion in most solid tumors. However, the roles of CAFs in the liver cancer microenvironment have not been thoroughly studied. In our previous study, we successfully isolated CAFs from hepatocellular carcinoma (HCC) (H-CAFs) and proved that H-CAFs suppressed the activation of NK cells and thereby created favorable conditio...

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Plos One : Cd2 Promotes Human Natural Killer Cell Membrane Nanotub...

By: Roland Jacobs

Description : Membrane nanotubes are thin membranous projections that physically connect two cells. While nanotubes have been studied in human natural killer (NK) cells and are implicated in aiding NK cell cytotoxic function, requirements for their formation to susceptible target cells remain incompletely understood. Here we demonstrate that the CD2-CD58/48 receptor-ligand interaction promotes and is required for nanotube formation in human NK cells. In the CD22 NK cell ...

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Plos One : Elevation of Eosinophil-derived Neurotoxin in Plasma of...

By: Helene F. Rosenberg

Description : Aspirin-exacerbated respiratory disease (AERD) remains widely underdiagnosed in asthmatics, primarily due to insufficient awareness of the relationship between aspirin ingestion and asthma exacerbation. The identification of aspirin hypersensitivity is therefore essential to avoid serious aspirin complications. The goal of the study was to develop plasma biomarkers to predict AERD. We identified differentially expressed genes in peripheral blood mononuclear...

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Plos One : White Blood Cell Counts as Risk Markers of Developing M...

By: Jose A. L. Calbet

Description : Total WBC counts, and some subtypes, were positively associated with MetS as well as hypertriglyceridemia, low HDL-cholesterol and high fasting glucose, all components of MetS.

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Plos One : Amino Acid Uptake in Arbuscular Mycorrhizal Plants, Vol...

By: Gustavo Bonaventure

Description : We examined the extent to which arbuscular mycorrhizal (AM) fungi root improved the acquisition of simple organic nitrogen (ON) compounds by their host plants. In a greenhouse-based study, we used quantum dots (fluorescent nanoparticles) to assess uptake of each of the 20 proteinaceous amino acids by AM-colonized versus uncolonized plants. We found that AM colonization increased uptake of phenylalanine, lysine, asparagine, arginine, histidine, methionine, t...

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Plos One : Visualization of Dc-sign-mediated Entry Pathway of Engi...

By: Jean-luc E.P.H. Darlix

Description : Dendritic cells (DCs) are potent antigen-presenting cells and therefore have enormous potential as vaccine targets. We have previously developed an engineered lentiviral vector (LV) that is pseudotyped with a mutated Sindbis virus glycoprotein (SVGmu), which is capable of targeting DCs through Dendritic Cell-specific ICAM3-grabbing Nonintegrin (DC-SIGN), a receptor that is predominantly expressed by DCs. In this study, we aimed to elucidate the internalizat...

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Plos One : Neural Dynamics of Attentional Cross-modality Control, ...

By: Gennady Cymbalyuk

Description : Attentional networks that integrate many cortical and subcortical elements dynamically control mental processes to focus on specific events and make a decision. The resources of attentional processing are finite. Nevertheless, we often face situations in which it is necessary to simultaneously process several modalities, for example, to switch attention between players in a soccer field. Here we use a global brain mode description to build a model of attent...

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Plos One : Novel Agents Targeting the Igf-1R, Volume 7

By: Javier S. Castresana

Description : The receptor tyrosine kinase (RTK)/phosphoinositide 3-kinase (PI3K) pathway is fundamental for cancer cell proliferation and is known to be frequently altered and activated in neoplasia, including embryonal tumors. Based on the high frequency of alterations, targeting components of the PI3K signaling pathway is considered to be a promising therapeutic approach for cancer treatment. Here, we have investigated the potential of targeting the axis of the insuli...

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Plos One : an Extension of Ppls-da for Classification and Comparis...

By: Kewei Chen

Description : Classification studies are widely applied, e.g. in biomedical research to classify objects/patients into predefined groups. The goal is to find a classification function/rule which assigns each object/patient to a unique group with the greatest possible accuracy (classification error). Especially in gene expression experiments often a lot of variables (genes) are measured for only few objects/patients. A suitable approach is the well-known method PLS-DA, wh...

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Plos One : Prospective Analysis of the Association of a Common Var...

By: Struan Frederick Airth Grant

Description : We confirmed the association between the FTO rs9939609 and body mass and overweight/obesity risk in European children. The main finding of the study is that the A allele carriers present higher increase of body mass and central adiposity over time and higher risk of developing overweight/obesity during growth, independently from intervention measures.

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Plos One : Interleukin 6 Deficiency Modulates the Hypothalamic Exp...

By: Raul M. Luque

Description : Pregnancy is associated with hyperphagia, increased adiposity and multiple neuroendocrine adaptations. Maternal adipose tissue secretes rising amounts of interleukin 6 (IL6), which acts peripherally modulating metabolic function and centrally increasing energy expenditure and reducing body fat. To explore the role of IL6 in the central mechanisms governing dam’s energy homeostasis, early, mid and late pregnant (gestational days 7, 13 and 18) wild-type (WT) ...

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Plos One : Tumor Burden Talks in Cancer Treatment with Pegylated L...

By: Chin-tu Chen

Description : The significant differences in the outcomes of cancer treatment and molecular imaging between animals bearing small and large tumors revealed that tumor burden is a critical and discriminative factor in cancer therapy using PEGylated liposomal drugs

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Plos One : Systematic Analysis of the Physiological Importance of ...

By: Brian D. McCabe

Description : Deubiquitinating enzymes (DUBs) are proteases that control the post-translational modification of proteins by ubiquitin and in turn regulate diverse cellular pathways. Despite a growing understanding of DUB biology at the structural and molecular level, little is known about the physiological importance of most DUBs. Here, we systematically identify DUBs encoded by the genome of Drosophila melanogaster and examine their physiological importance in vivo. Thr...

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Plos One : Analysis of the Clonality of Candida Tropicalis Strains...

By: Wenjun Li

Description : Multilocus sequence typing (MLST) based on six loci was used to analyze the relationship of 58 Candida tropicalis isolates from individual patients in a general hospital in Beijing, China. A total of 52 diploid sequence types (DSTs) were generated by the MLST, all of which were new to the central database. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrograms were constructed, which showed that the 58 isolates were distributed robustly and 6...

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Plos One : Attachment Styles and Personal Growth Following Romanti...

By: Marianna Mazza

Description : The purpose of this research was to examine the associations of attachment anxiety and avoidance with personal growth following relationship dissolution, and to test breakup distress, rumination, and tendency to rebound with new partners as mediators of these associations. Study 1 (N = 411) and Study 2 (N = 465) measured attachment style, breakup distress, and personal growth: Study 2 additionally measured ruminative reflection, brooding, and proclivity to ...

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