World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Biology : the Chromosomal Passenger Complex Activates Polo Kinase at Centromeres, Volume 10

By Hawley, R. Scott

Click here to view

Book Id: WPLBN0003922417
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Biology : the Chromosomal Passenger Complex Activates Polo Kinase at Centromeres, Volume 10  
Author: Hawley, R. Scott
Volume: Volume 10
Language: English
Subject: Journals, Science, Biology
Collections: Periodicals: Journal and Magazine Collection (Contemporary), PLoS Biology
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Hawley, R. S. (n.d.). Plos Biology : the Chromosomal Passenger Complex Activates Polo Kinase at Centromeres, Volume 10. Retrieved from http://netlibrary.net/


Description
Description : The coordinated activities at centromeres of two key cell cycle kinases, Polo and Aurora B, are critical for ensuring that the two sister kinetochores of each chromosome are attached to microtubules from opposite spindle poles prior to chromosome segregation at anaphase. Initial attachments of chromosomes to the spindle involve random interactions between kinetochores and dynamic microtubules, and errors occur frequently during early stages of the process. The balance between microtubule binding and error correction (e.g., release of bound microtubules) requires the activities of Polo and Aurora B kinases, with Polo promoting stable attachments and Aurora B promoting detachment. Our study concerns the coordination of the activities of these two kinases in vivo. We show that INCENP, a key scaffolding subunit of the chromosomal passenger complex (CPC), which consists of Aurora B kinase, INCENP, Survivin, and Borealin/Dasra B, also interacts with Polo kinase in Drosophila cells. It was known that Aurora A/Bora activates Polo at centrosomes during late G2. However, the kinase that activates Polo on chromosomes for its critical functions at kinetochores was not known. We show here that Aurora B kinase phosphorylates Polo on its activation loop at the centromere in early mitosis. This phosphorylation requires both INCENP and Aurora B activity (but not Aurora A activity) and is critical for Polo function at kinetochores. Our results demonstrate clearly that Polo kinase is regulated differently at centrosomes and centromeres and suggest that INCENP acts as a platform for kinase crosstalk at the centromere. This crosstalk may enable Polo and Aurora B to achieve a balance wherein microtubule mis-attachments are corrected, but proper attachments are stabilized allowing proper chromosome segregation.

 

Click To View

Additional Books


  • Plos Biology : Whole Animal Experiments ... (by )
  • Plos Biology : Pulsed Feedback Defers Ce... (by )
  • Plos Biology : West Nile Virus Epidemics... (by )
  • Plos Biology : Algorithmic Self-assembly... (by )
  • Plos Biology : a Conserved Supergene Loc... (by )
  • Plos Biology : Fog-2 and the Evolution o... (by )
  • Plos Biology : Mrna Secondary Structures... (by )
  • Plos Biology : Modeling the Mutualistic ... (by )
  • Plos Biology : How Hiv Sneaks Aboard Mat... (by )
  • Plos Biology : Aging in a Long-lived Clo... (by )
  • Plos Biology : Distributive Conjugal Tra... (by )
  • Plos Biology : Pax7 is Necessary and Suf... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.