World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Biology : High-resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast, Volume 4

By Wolfe, Kenneth

Click here to view

Book Id: WPLBN0003925398
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Biology : High-resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast, Volume 4  
Author: Wolfe, Kenneth
Volume: Volume 4
Language: English
Subject: Journals, Science, Biology
Collections: Periodicals: Journal and Magazine Collection, PLoS Biology
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Wolfe, K. (n.d.). Plos Biology : High-resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast, Volume 4. Retrieved from http://netlibrary.net/


Description
Description : Understanding the genetic basis of evolutionary adaptation is limited by our ability to efficiently identify the genomic locations of adaptive mutations. Here we describe a method that can quickly and precisely map the genetic basis of naturally and experimentally evolved complex traits using linkage analysis. A yeast strain that expresses the evolved trait is crossed to a distinct strain background and DNA from a large pool of progeny that express the trait of interest is hybridized to oligonucleotide microarrays that detect thousands of polymorphisms between the two strains. Adaptive mutations are detected by linkage to the polymorphisms from the evolved parent. We successfully tested our method by mapping five known genes to a precision of 0.2–24 kb (0.1–10 cM), and developed computer simulations to test the effect of different factors on mapping precision. We then applied this method to four yeast strains that had independently adapted to a fluctuating glucose–galactose environment. All four strains had acquired one or more missense mutations in GAL80, the repressor of the galactose utilization pathway. When transferred into the ancestral strain, the gal80 mutations conferred the fitness advantage that the evolved strains show in the transition from glucose to galactose. Our results show an example of parallel adaptation caused by mutations in the same gene.

 

Click To View

Additional Books


  • Plos Biology : Quality Information for I... (by )
  • Plos Biology : Light Activates Output fr... (by )
  • Plos Biology : the Gene Vitellogenin Has... (by )
  • Plos Biology : Whole-genome Analysis of ... (by )
  • Plos Biology : Cryptic Variation in the ... (by )
  • Plos Biology : Replication Publication, ... (by )
  • Plos Biology : Intracellular Proton Acce... (by )
  • Plos Biology : Theta Rhythms Coordinate ... (by )
  • Plos Biology : Multiple Apoptotic Caspas... (by )
  • Plos Biology : Atonal Homolog 1 is a Tum... (by )
  • Plos Biology : Echolocating Bats Use a N... (by )
  • Plos Biology : Pheromonal and Behavioral... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.