World Library  

Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Biology : MacRonuclear Genome Sequence of the Ciliate Tetrahymena Thermophila, a Model Eukaryote, Volume 4

By Gelfand, Mikhail

Click here to view

Book Id: WPLBN0003925432
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Biology : MacRonuclear Genome Sequence of the Ciliate Tetrahymena Thermophila, a Model Eukaryote, Volume 4  
Author: Gelfand, Mikhail
Volume: Volume 4
Language: English
Subject: Journals, Science, Biology
Collections: Periodicals: Journal and Magazine Collection, PLoS Biology
Publication Date:
Publisher: Plos


APA MLA Chicago

Gelfand, M. (n.d.). Plos Biology : MacRonuclear Genome Sequence of the Ciliate Tetrahymena Thermophila, a Model Eukaryote, Volume 4. Retrieved from

Description : The ciliate Tetrahymena thermophila is a model organism for molecular and cellular biology. Like other ciliates, this species has separate germline and soma functions that are embodied by distinct nuclei within a single cell. The germline-like micronucleus (MIC) has its genome held in reserve for sexual reproduction. The soma-like macronucleus (MAC), which possesses a genome processed from that of the MIC, is the center of gene expression and does not directly contribute DNA to sexual progeny. We report here the shotgun sequencing, assembly, and analysis of the MAC genome of T. thermophila, which is approximately 104 Mb in length and composed of approximately 225 chromosomes. Overall, the gene set is robust, with more than 27,000 predicted protein-coding genes, 15,000 of which have strong matches to genes in other organisms. The functional diversity encoded by these genes is substantial and reflects the complexity of processes required for a free-living, predatory, single-celled organism. This is highlighted by the abundance of lineage-specific duplications of genes with predicted roles in sensing and responding to environmental conditions (e.g., kinases), using diverse resources (e.g., proteases and transporters), and generating structural complexity (e.g., kinesins and dyneins). In contrast to the other lineages of alveolates (apicomplexans and dinoflagellates), no compelling evidence could be found for plastid-derived genes in the genome. UGA, the only T. thermophila stop codon, is used in some genes to encode selenocysteine, thus making this organism the first known with the potential to translate all 64 codons in nuclear genes into amino acids. We present genomic evidence supporting the hypothesis that the excision of DNA from the MIC to generate the MAC specifically targets foreign DNA as a form of genome self-defense. The combination of the genome sequence, the functional diversity encoded therein, and the presence of some pathways missing from other model organisms makes T. thermophila an ideal model for functional genomic studies to address biological, biomedical, and biotechnological questions of fundamental importance.


Click To View

Additional Books

  • Plos Biology : Trophic Complexity and th... (by )
  • Plos Biology : Quantitative Control of O... (by )
  • Plos Biology : Tolerance to Self ; Which... (by )
  • Plos Biology : Novel Melatonin-blocking ... (by )
  • Plos Biology : an Acetylcholine Receptor... (by )
  • Plos Biology : a Katp Channel-dependent ... (by )
  • Plos Biology : Cftr Delivery to 25% of S... (by )
  • Plos Biology : Antagonism of Lin-17, Vol... (by )
  • Plos Biology : Light, Sleep, and Circadi... (by )
  • Plos Biology : Filling in the Gaps ; Art... (by )
  • Plos Biology : Humans Lack Igb3 Due to t... (by )
  • Plos Biology : Mapping Meiotic Single-st... (by )
Scroll Left
Scroll Right


Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.