World Library  

Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Biology : Dynamics and Design Principles of a Basic Regulatory Architecture Controlling Metabolic Pathways, Volume 6

By Chin, Chen-shan

Click here to view

Book Id: WPLBN0003928804
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Biology : Dynamics and Design Principles of a Basic Regulatory Architecture Controlling Metabolic Pathways, Volume 6  
Author: Chin, Chen-shan
Volume: Volume 6
Language: English
Subject: Journals, Science, Biology
Collections: Periodicals: Journal and Magazine Collection, PLoS Biology
Publication Date:
Publisher: Plos


APA MLA Chicago

Chin, C. (n.d.). Plos Biology : Dynamics and Design Principles of a Basic Regulatory Architecture Controlling Metabolic Pathways, Volume 6. Retrieved from

Description : The dynamic features of a genetic network’s response to environmental fluctuations represent essential functional specifications and thus may constrain the possible choices of network architecture and kinetic parameters. To explore the connection between dynamics and network design, we have analyzed a general regulatory architecture that is commonly found in many metabolic pathways. Such architecture is characterized by a dual control mechanism, with end product feedback inhibition and transcriptional regulation mediated by an intermediate metabolite. As a case study, we measured with high temporal resolution the induction profiles of the enzymes in the leucine biosynthetic pathway in response to leucine depletion, using an automated system for monitoring protein expression levels in single cells. All the genes in the pathway are known to be coregulated by the same transcription factors, but we observed drastically different dynamic responses for enzymes upstream and immediately downstream of the key control point—the intermediate metabolite a-isopropylmalate (aIPM), which couples metabolic activity to transcriptional regulation. Analysis based on genetic perturbations suggests that the observed dynamics are due to differential regulation by the leucine branch-specific transcription factor Leu3, and that the downstream enzymes are strictly controlled and highly expressed only when aIPM is available. These observations allow us to build a simplified mathematical model that accounts for the observed dynamics and can correctly predict the pathway’s response to new perturbations. Our model also suggests that transient dynamics and steady state can be separately tuned and that the high induction levels of the downstream enzymes are necessary for fast leucine recovery. It is likely that principles emerging from this work can reveal how gene regulation has evolved to optimize performance in other metabolic pathways with similar architecture.


Click To View

Additional Books

  • Plos Biology : Active Control of Acousti... (by )
  • Plos Biology : a Widespread Chromosomal ... (by )
  • Plos Biology : Evolution-based Drug Disc... (by )
  • Plos Biology : the Bittersweet Sounds of... (by )
  • Plos Biology : Structural and Chemical P... (by )
  • Plos Biology : Connecting Variability in... (by )
  • Plos Biology : Gene Gain and Loss During... (by )
  • Plos Biology : Equity for Open-access Jo... (by )
  • Plos Biology : a Competition Between Sti... (by )
  • Plos Biology : Opposing Nodal and Bmp Si... (by )
  • Plos Biology : Speciation and Gene Flow ... (by )
  • Plos Biology : in Plos Biology, Volume 2... (by )
Scroll Left
Scroll Right


Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.