World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Biology : in Silico Reconstitution of Actin-based Symmetry Breaking and Motility, Volume 7

By Pollard, Thomas

Click here to view

Book Id: WPLBN0003930790
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Biology : in Silico Reconstitution of Actin-based Symmetry Breaking and Motility, Volume 7  
Author: Pollard, Thomas
Volume: Volume 7
Language: English
Subject: Journals, Science, Biology
Collections: Periodicals: Journal and Magazine Collection, PLoS Biology
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Pollard, T. (n.d.). Plos Biology : in Silico Reconstitution of Actin-based Symmetry Breaking and Motility, Volume 7. Retrieved from http://netlibrary.net/


Description
Description : Eukaryotic cells assemble viscoelastic networks of crosslinked actin filaments to control their shape, mechanical properties, and motility. One important class of actin network is nucleated by the Arp2/3 complex and drives both membrane protrusion at the leading edge of motile cells and intracellular motility of pathogens such as Listeria monocytogenes. These networks can be reconstituted in vitro from purified components to drive the motility of spherical micron-sized beads. An Elastic Gel model has been successful in explaining how these networks break symmetry, but how they produce directed motile force has been less clear. We have combined numerical simulations with in vitro experiments to reconstitute the behavior of these motile actin networks in silico using an Accumulative Particle-Spring (APS) model that builds on the Elastic Gel model, and demonstrates simple intuitive mechanisms for both symmetry breaking and sustained motility. The APS model explains observed transitions between smooth and pulsatile motion as well as subtle variations in network architecture caused by differences in geometry and conditions. Our findings also explain sideways symmetry breaking and motility of elongated beads, and show that elastic recoil, though important for symmetry breaking and pulsatile motion, is not necessary for smooth directional motility. The APS model demonstrates how a small number of viscoelastic network parameters and construction rules suffice to recapture the complex behavior of motile actin networks. The fact that the model not only mirrors our in vitro observations, but also makes novel predictions that we confirm by experiment, suggests that the model captures much of the essence of actin-based motility in this system.

 

Click To View

Additional Books


  • Plos Biology : Conservation and Evolutio... (by )
  • Plos Biology : Liver is T Cells’ Ace in ... (by )
  • Plos Biology : Nox4 ; a Guilty Party in ... (by )
  • Plos Biology : Control of Transcription ... (by )
  • Plos Biology : Multi-cellular Rosettes i... (by )
  • Plos Biology : Pax7 is Necessary and Suf... (by )
  • Plos Biology : Differentiation Driven Ch... (by )
  • Plos Biology : Grasping the Intentions o... (by )
  • Plos Biology : Spontaneous Autoimmunity ... (by )
  • Plos Biology : Genomic Islands of Specia... (by )
  • Plos Biology : Object-oriented Echo Perc... (by )
  • Plos Biology : Science Incubators ; Synt... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.