World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Biology : the Hierarchy of Exon-junction Complex Assembly by the Spliceosome Explains Key Features of Mammalian Nonsense-mediated Mrna Decay, Volume 7

By Misteli, Tom

Click here to view

Book Id: WPLBN0003930894
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Biology : the Hierarchy of Exon-junction Complex Assembly by the Spliceosome Explains Key Features of Mammalian Nonsense-mediated Mrna Decay, Volume 7  
Author: Misteli, Tom
Volume: Volume 7
Language: English
Subject: Journals, Science, Biology
Collections: Periodicals: Journal and Magazine Collection, PLoS Biology
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Misteli, T. (n.d.). Plos Biology : the Hierarchy of Exon-junction Complex Assembly by the Spliceosome Explains Key Features of Mammalian Nonsense-mediated Mrna Decay, Volume 7. Retrieved from http://netlibrary.net/


Description
Description : Exon junction complexes (EJCs) link nuclear splicing to key features of mRNA function including mRNA stability, translation, and localization. We analyzed the formation of EJCs by the spliceosome, the physiological EJC assembly machinery. We studied a comprehensive set of eIF4A3, MAGOH, and BTZ mutants in complete or C-complex–arrested splicing reactions and identified essential interactions of EJC proteins during and after EJC assembly. These data establish that EJC deposition proceeds through a defined intermediate, the pre-EJC, as an ordered, sequential process that is coordinated by splicing. The pre-EJC consists of eIF4A3 and MAGOH-Y14, is formed before exon ligation, and provides a binding platform for peripheral EJC components that join after release from the spliceosome and connect the core structure with function. Specifically, we identified BTZ to bridge the EJC to the nonsense-mediated messenger RNA (mRNA) decay protein UPF1, uncovering a critical link between mRNP architecture and mRNA stability. Based on this systematic analysis of EJC assembly by the spliceosome, we propose a model of how a functional EJC is assembled in a strictly sequential and hierarchical fashion, including nuclear splicing-dependent and cytoplasmic steps.

 

Click To View

Additional Books


  • Plos Biology : Sir2 Paralogues Cooperate... (by )
  • Plos Biology : Mapping Meiotic Single-st... (by )
  • Plos Biology : Patterns and Mechanisms o... (by )
  • Plos Biology : Why Plos Became a Publish... (by )
  • Plos Biology : How to Make Epidemiologic... (by )
  • Plos Biology : Dorsoventral Patterning i... (by )
  • Plos Biology : Fabp7 Maps to a Quantitat... (by )
  • Plos Biology : Neuronal Oscillations Enh... (by )
  • Plos Biology : Redefining “natural” in A... (by )
  • Plos Biology : Mglb Fills a Gap in Bacte... (by )
  • Plos Biology : Malaria Infection Increas... (by )
  • Plos Biology : Hyphal Development in Can... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.