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Plos One : a Heat-shock Protein Axis Regulates Vegfr2 Proteolysis, Blood Vessel Development and Repair, Volume 7

By Madeddu, Paolo

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Book Id: WPLBN0003932803
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos One : a Heat-shock Protein Axis Regulates Vegfr2 Proteolysis, Blood Vessel Development and Repair, Volume 7  
Author: Madeddu, Paolo
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

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Madeddu, P. (n.d.). Plos One : a Heat-shock Protein Axis Regulates Vegfr2 Proteolysis, Blood Vessel Development and Repair, Volume 7. Retrieved from http://netlibrary.net/


Description
Description : Vascular endothelial growth factor A (VEGF-A) binds to the VEGFR2 receptor tyrosine kinase, regulating endothelial function, vascular physiology and angiogenesis. However, the mechanism underlying VEGFR2 turnover and degradation in this response is unclear. Here, we tested a role for heat-shock proteins in regulating the presentation of VEGFR2 to a degradative pathway. Pharmacological inhibition of HSP90 stimulated VEGFR2 degradation in primary endothelial cells and blocked VEGF-A-stimulated intracellular signaling via VEGFR2. HSP90 inhibition stimulated the formation of a VEGFR2-HSP70 complex. Clathrin-mediated VEGFR2 endocytosis is required for this HSP-linked degradative pathway for targeting VEGFR2 to the endosome-lysosome system. HSP90 perturbation selectively inhibited VEGF-A-stimulated human endothelial cell migration in vitro. A mouse femoral artery model showed that HSP90 inhibition also blocked blood vessel repair in vivo consistent with decreased endothelial regeneration. Depletion of either HSP70 or HSP90 caused defects in blood vessel formation in a transgenic zebrafish model. We conclude that perturbation of the HSP70-HSP90 heat-shock protein axis stimulates degradation of endothelial VEGFR2 and modulates VEGF-A-stimulated intracellular signaling, endothelial cell migration, blood vessel development and repair.

 

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