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Plos One : Application of On-line Nanolc-it-tof in the Identification of Serum Β-catenin Complex in Mice Scald Model, Volume 7

By Katoh, Masaru

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Book Id: WPLBN0003933970
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos One : Application of On-line Nanolc-it-tof in the Identification of Serum Β-catenin Complex in Mice Scald Model, Volume 7  
Author: Katoh, Masaru
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection
Historic
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Publisher: Plos

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Katoh, M. (n.d.). Plos One : Application of On-line Nanolc-it-tof in the Identification of Serum Β-catenin Complex in Mice Scald Model, Volume 7. Retrieved from http://netlibrary.net/


Description
Description : Severe burn shock remains an unresolved clinical problem with an urgent need to explore novel therapeutic treatments. Intracellular b-catenin, through interaction with other proteins, has been reported to be able to regulate the size of cutaneous wounds. Higher expression of b-catenin is associated with larger sized wounds. However, the identification of serum b-catenin complex is difficult and has been rarely reported. The exploitation of more binding partners can contribute to uncovering the exact mechanisms behind serum b-catenin mediated biological effects. Here, we describe a method that consists of immunoprecipitation, SDS-PAGE, in-gel digestion, and nanoLC coupled to LCMS-IT-TOF for the investigation of serum b-catenin complex in mice scald model. Among selected gel bands obtained from the protein gels, a total of 31 peptides were identified and sequenced with high statistical significance (p,0.01). Three proteins (alpha-2-marcoglobulin, serine protease inhibitor A3K, and serine protease inhibitor A1A) were identified and validated with high reliability and high reproducibility. It was inferred that these proteins might interact with serum b-catenin, which could affect the wound healing resulting from burn shock. Our study demonstrated that the on-line coupling of nano-LC with a LCMS-IT-TOF mass spectrometer was capable of sensitive and automated characterization of the serum b-catenin complex in mice scald model

 

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