World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Biochemical and Mutational Analysis of a Novel Nicotinamidase from Oceanobacillus Iheyensis Hte831, Volume 7

By Appanna, Vasu, D.

Click here to view

Book Id: WPLBN0003934415
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Biochemical and Mutational Analysis of a Novel Nicotinamidase from Oceanobacillus Iheyensis Hte831, Volume 7  
Author: Appanna, Vasu, D.
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Appanna, V. D. (n.d.). Plos One : Biochemical and Mutational Analysis of a Novel Nicotinamidase from Oceanobacillus Iheyensis Hte831, Volume 7. Retrieved from http://netlibrary.net/


Description
Description : Nicotinamidases catalyze the hydrolysis of nicotinamide to nicotinic acid and ammonia, an important reaction in the NAD+ salvage pathway. This paper reports a new nicotinamidase from the deep-sea extremely halotolerant and alkaliphilic Oceanobacillus iheyensis HTE831 (OiNIC). The enzyme was active towards nicotinamide and several analogues, including the prodrug pyrazinamide. The enzyme was more nicotinamidase (kcat/Km = 43.5 mM21s21) than pyrazinamidase (kcat/ Km = 3.2 mM21s21). Mutational analysis was carried out on seven critical amino acids, confirming for the first time the importance of Cys133 and Phe68 residues for increasing pyrazinamidase activity 2.9- and 2.5-fold, respectively. In addition, the change in the fourth residue involved in the ion metal binding (Glu65) was detrimental to pyrazinamidase activity, decreasing it 6-fold. This residue was also involved in a new distinct structural motif DAHXXXDXXHPE described in this paper for Firmicutes nicotinamidases. Phylogenetic analysis revealed that OiNIC is the first nicotinamidase described for the order Bacillales.

 

Click To View

Additional Books


  • Plos One : Evolutionary Characterization... (by )
  • Plos One : Pleiotrophin Gene Therapy for... (by )
  • Plos One : Wavelength Optimization for Q... (by )
  • Plos One : Degradation of Muc7 and Muc5B... (by )
  • Plos One : Cmyc, Volume 8 (by )
  • Plos One : Influence of Sire Breed on th... (by )
  • Plos One : Development and Verification ... (by )
  • Plos One : Regulation of Retinoid-mediat... (by )
  • Plos One : Epidemiological Analysis of H... (by )
  • Plos One : Long-term Treatment of Thalid... (by )
  • Plos One : Crest – Classification Resour... (by )
  • Plos One : Trophic Interactions and Dist... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.