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Plos One : Early-life Gut Bacteria Associate with Il-42, Il-102 and Ifn-γ Production at Two Years of Age, Volume 7

By Sanz, Yolanda

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Book Id: WPLBN0003936677
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos One : Early-life Gut Bacteria Associate with Il-42, Il-102 and Ifn-γ Production at Two Years of Age, Volume 7  
Author: Sanz, Yolanda
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

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Sanz, Y. (n.d.). Plos One : Early-life Gut Bacteria Associate with Il-42, Il-102 and Ifn-γ Production at Two Years of Age, Volume 7. Retrieved from http://netlibrary.net/


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Description : Microbial exposure early in life influences immune maturation and potentially also the development of immune-mediated disease. Here we studied early-life gut colonization in relation to cytokine responses at two years of age. Fecal samples were collected from infants during the first two months of life. DNA was extracted from the fecal samples and Bifidobacterium (B.) adolescentis, B. breve, B. bifidum, a group of lactobacilli (L. casei, L. paracasei and L. rhamnosus) as well as Staphylococcus (S.) aureus were detected with real time PCR. Peripheral mononuclear cells were stimulated with phytohaemagglutinin (PHA) and numbers of IL-42, IL-102 and IFN-c secreting cells were evaluated using ELISpot. We further stimulated peripheral blood mononuclear cells with bacterial supernatants in vitro and assessed the IL-42, IL-102 and IFN-c inducing capacity by flow cytometry and ELISA. Early S. aureus colonization associated with higher numbers of IL-42 (p = 0.022) and IL-10 (p = 0.016) producing cells at two years of age. In contrast to colonization with S. aureus alone, co-colonization with lactobacilli associated with suppression of IL-42 (p = 0.004), IL-102 (p = 0.004) and IFN-c (p = 0.034) secreting cells. In vitro stimulations of mononuclear cells with bacterial supernatants supported a suppressive role of L. rhamnosus GG on S. aureusinduced cytokine responses. We demonstrate that the early gut colonization pattern associates with the PHA-induced cytokine profile at two years of age and our in vitro findings support that specific bacterial species influence the T helper cell subsets. This suggests that dysbiosis in the early microbiota may modulate the risk of developing inflammatory conditions like allergy.

 

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