World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Functional Analyses of Complement Convertases Using C3 and C5-depleted Sera, Volume 7

By Hollander, Anneke I. Den

Click here to view

Book Id: WPLBN0003937826
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Functional Analyses of Complement Convertases Using C3 and C5-depleted Sera, Volume 7  
Author: Hollander, Anneke I. Den
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Den Hollander, A. I. (n.d.). Plos One : Functional Analyses of Complement Convertases Using C3 and C5-depleted Sera, Volume 7. Retrieved from http://netlibrary.net/


Description
Description : C3 and C5 convertases are central stages of the complement cascade since they converge the different initiation pathways, augment complement activation by an amplification loop and lead to a common terminal pathway resulting in the formation of the membrane attack complex. Several complement inhibitors attenuate convertase formation and/or accelerate dissociation of convertase complexes. Functional assays used to study these processes are often performed using purified complement components, from which enzymatic complexes are reconstituted on the surface of erythrocytes or artificial matrices. This strategy enables identification of individual interactions between convertase components and putative regulators but carries an inherent risk of detecting non-physiological interactions that would not occur in a milieu of whole serum. Here we describe a novel, alternative method based on C3 or C5-depleted sera, which support activation of the complement cascade up to the desired stages of convertases. This approach allows fast and simple assessment of the influence of putative regulators on convertase formation and stability. As an example of practical utility of the assay, we performed studies on thioredoxin-1 in order to clarify the mechanism of its influence on complement convertases.

 

Click To View

Additional Books


  • Plos One : Identification and Validation... (by )
  • Plos One : Protein Disulfide Isomerase-l... (by )
  • Plos One : Unique Kinase Catalytic Mecha... (by )
  • Plos One : Revisiting the Two-layer Hypo... (by )
  • Plos One : Cellular and Cytokine Correla... (by )
  • Plos One : Activity of Fusion Prophenolo... (by )
  • Plos One : a Refined Study of Fcrl Genes... (by )
  • Plos One : Change and Variability in Eas... (by )
  • Plos One : the Ratio of Treg, Volume 7 (by )
  • Plos One : Health and Economic Burden of... (by )
  • Plos One : Presence of Hydrogen Peroxide... (by )
  • Plos One : Transcriptional Regulation of... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.