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Plos One : Housekeeping Gene Selection Advisory ; Glyceraldehyde- 3-phosphate Dehydrogenase Gapdh and Β-actin Are Targets of Mir-644A, Volume 7

By Stoecklin, Georg

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Book Id: WPLBN0003938678
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos One : Housekeeping Gene Selection Advisory ; Glyceraldehyde- 3-phosphate Dehydrogenase Gapdh and Β-actin Are Targets of Mir-644A, Volume 7  
Author: Stoecklin, Georg
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
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Publisher: Plos

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Stoecklin, G. (n.d.). Plos One : Housekeeping Gene Selection Advisory ; Glyceraldehyde- 3-phosphate Dehydrogenase Gapdh and Β-actin Are Targets of Mir-644A, Volume 7. Retrieved from http://netlibrary.net/


Description
Description : Results of overexpression or downregulation of a microRNA (miRNA) on its target mRNA expression are often validated by reverse-transcription and quantitative PCR analysis using an appropriate housekeeping gene as an internal control. The possible direct or indirect effects of a miRNA on the expression of housekeeping genes are often overlooked. Among many housekeeping genes, expressions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and b-actin have been used extensively for normalization of gene expression data. Here, we show that GAPDH and b-actin are direct targets of miR- 644a. Our data demonstrate the unsuitability of GAPDH and b-actin as internal controls in miR-644a functional studies and emphasize the need to carefully consider the choice of a reference gene in miRNA experiments.

 

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