World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Mepe-derived Asarm Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia, Volume 7

By Kerkis, Irina

Click here to view

Book Id: WPLBN0003940544
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Mepe-derived Asarm Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia, Volume 7  
Author: Kerkis, Irina
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Kerkis, I. (n.d.). Plos One : Mepe-derived Asarm Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia, Volume 7. Retrieved from http://netlibrary.net/


Description
Description : Mutations in PHEX (phosphate-regulating gene with homologies to endopeptidases on the X-chromosome) cause X-linked familial hypophosphatemic rickets (XLH), a disorder having severe bone and tooth dentin mineralization defects. The absence of functional PHEX leads to abnormal accumulation of ASARM (acidic serine- and aspartate-rich motif) peptide 2 a substrate for PHEX and a strong inhibitor of mineralization 2 derived from MEPE (matrix extracellular phosphoglycoprotein) and other matrix proteins. MEPE-derived ASARM peptide accumulates in tooth dentin of XLH patients where it may impair dentinogenesis. Here, we investigated the effects of ASARM peptides in vitro and in vivo on odontoblast differentiation and matrix mineralization. Dental pulp stem cells from human exfoliated deciduous teeth (SHEDs) were seeded into a 3D collagen scaffold, and induced towards odontogenic differentiation. Cultures were treated with synthetic ASARM peptides (phosphorylated and nonphosphorylated) derived from the human MEPE sequence. Phosphorylated ASARM peptide inhibited SHED differentiation in vitro, with no mineralized nodule formation, decreased odontoblast marker expression, and upregulated MEPE expression. Phosphorylated ASARM peptide implanted in a rat molar pulp injury model impaired reparative dentin formation and mineralization, with increased MEPE immunohistochemical staining. In conclusion, using complementary models to study tooth dentin defects observed in XLH, we demonstrate that the MEPE-derived ASARM peptide inhibits both odontogenic differentiation and matrix mineralization, while increasing MEPE expression. These results contribute to a partial mechanistic explanation of XLH pathogenesis : direct inhibition of mineralization by ASARM peptide leads to the mineralization defects in XLH teeth. This process appears to be positively reinforced by the increased MEPE expression induced by ASARM. The MEPE-ASARM system can therefore be considered as a potential therapeutic target.

 

Click To View

Additional Books


  • Plos One : Phylogeography of Bivalve Cyc... (by )
  • Plos One : Early-life Gut Bacteria Assoc... (by )
  • Plos One : Innovative Techniques for Est... (by )
  • Plos One : Sarcoptes Scabiei Mites Modul... (by )
  • Plos One : Diversity and Complexity in C... (by )
  • Plos One : Pharmacogenetic Analysis of P... (by )
  • Plos One : Population Genetic Structure ... (by )
  • Plos One : Pnpla3 Gg Genotype and Caroti... (by )
  • Plos One : Connectivity Between Migratin... (by )
  • Plos One : P-selectin Glycoprotein Ligan... (by )
  • Plos One : Phylogenetic Analysis of Micr... (by )
  • Plos One : Gnps-cs, Volume 8 (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.