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Plos One : Metabolite Profiling of a Diverse Collection of Wheat Lines Using Ultraperformance Liquid Chromatography Coupled with Time-of-flight Mass Spectrometry, Volume 7

By Subramanyam, Rajagopal

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Book Id: WPLBN0003940588
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos One : Metabolite Profiling of a Diverse Collection of Wheat Lines Using Ultraperformance Liquid Chromatography Coupled with Time-of-flight Mass Spectrometry, Volume 7  
Author: Subramanyam, Rajagopal
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
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Publisher: Plos

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Subramanyam, R. (n.d.). Plos One : Metabolite Profiling of a Diverse Collection of Wheat Lines Using Ultraperformance Liquid Chromatography Coupled with Time-of-flight Mass Spectrometry, Volume 7. Retrieved from http://netlibrary.net/


Description
Description : Genetic differences among major types of wheat are well characterized: however, little is known about how these distinctions affect the small molecule profile of the wheat seed. Ethanol/water (65% v/v) extracts of seed from 45 wheat lines representing 3 genetically distinct classes, tetraploid durum (Triticum turgidum subspecies durum) (DW) and hexaploid hard and soft bread wheat (T. aestivum subspecies aestivum) (BW) were subjected to ultraperformance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-TOF-MS). Discriminant analyses distinguished DW from BW with 100% accuracy due to differences in expression of nonpolar and polar ions, with differences attributed to sterol lipids/fatty acids and phospholipids/glycerolipids, respectively. Hard versus soft BW was distinguished with 100% accuracy by polar ions, with differences attributed to heterocyclic amines and polyketides versus phospholipid ions, respectively. This work provides a foundation for identification of metabolite profiles associated with desirable agronomic and human health traits and for assessing how environmental factors impact these characteristics.

 

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