World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : a Simple Method to Extract Dna from Hair Shafts Using Enzymatic Laundry Powder, Volume 8

By Gilbert, Tom

Click here to view

Book Id: WPLBN0003943088
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : a Simple Method to Extract Dna from Hair Shafts Using Enzymatic Laundry Powder, Volume 8  
Author: Gilbert, Tom
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Gilbert, T. (n.d.). Plos One : a Simple Method to Extract Dna from Hair Shafts Using Enzymatic Laundry Powder, Volume 8. Retrieved from http://netlibrary.net/


Description
Description : A simple method to extract DNA from hair shafts was developed by using enzymatic laundry powder at the first step of the process. The whole extraction can be finished in less than 2 hours. The simple extraction reagent proposed here contains only two cheap components : ordinary enzymatic laundry powder and PCR buffer. After extraction, an ultra sensitive fluorescent nucleic acid stain, PicoGreen, was used for quantifying trace amount of double-stranded DNA in the solution extracted. For further validation of DNA extraction, four primers were employed to amplify DNA microsatellite loci. Both fluorescence spectroscopy and PCR results suggested that this method can extract DNA from hair shafts with good efficiency and repeatability. The study will greatly facilitate the use of hair shafts in future for DNA analyses on genome-wide scale.


 

Click To View

Additional Books


  • Plos One : Frequency of Discrimination, ... (by )
  • Plos One : Women Versus Men with Chronic... (by )
  • Plos One : a Yeast Purification System f... (by )
  • Plos One : Effects of Multi-deficiencies... (by )
  • Plos One : Structure and Genetic Content... (by )
  • Plos One : Bacterial Cyclic Amp-phosphod... (by )
  • Plos One : Phylogeny as a Proxy for Ecol... (by )
  • Plos One : Aureolib — a Proteome Signatu... (by )
  • Plos One : Determination of Dominant Fre... (by )
  • Plos One : Long-term Impact of Earthquak... (by )
  • Plos One : Critical Role of Heat Shock P... (by )
  • Plos One : Perinatal Na+ Overload Progra... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.