World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Augmentation of Antitumor Immunity by Fusions of Ethanol-treated Tumor Cells and Dendritic Cells Stimulated Via Dual Tlrs Through Tgf-b1 Blockade and Il-12P70 Production, Volume 8

By Appel, Silke

Click here to view

Book Id: WPLBN0003944374
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Augmentation of Antitumor Immunity by Fusions of Ethanol-treated Tumor Cells and Dendritic Cells Stimulated Via Dual Tlrs Through Tgf-b1 Blockade and Il-12P70 Production, Volume 8  
Author: Appel, Silke
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Appel, S. (n.d.). Plos One : Augmentation of Antitumor Immunity by Fusions of Ethanol-treated Tumor Cells and Dendritic Cells Stimulated Via Dual Tlrs Through Tgf-b1 Blockade and Il-12P70 Production, Volume 8. Retrieved from http://netlibrary.net/


Description
Description : The therapeutic efficacy of fusion cell (FC)-based cancer vaccine generated with whole tumor cells and dendritic cells (DCs) requires the improved immunogenicity of both cells. Treatment of whole tumor cells with ethanol resulted in blockade of immune-suppressive soluble factors such as transforming growth factor (TGF)-b1, vascular endothelial growth factor, and IL- 10 without decreased expression of major histocompatibility complex (MHC) class I and the MUC1 tumor-associated antigen. Moreover, the ethanol-treated tumor cells expressed ‘‘eat-me’’ signals such as calreticulin (CRT) on the cell surface and released immunostimulatory factors such as heat shock protein (HSP)90a and high-mobility group box 1 (HMGB1). A dual stimulation of protein-bound polysaccharides isolated from Coriolus versicolor (TLR2 agonist) and penicillin-inactivated Streptococcus pyogenes (TLR4 agonist) led human monocyte-derived DCs to produce HSP90a and multiple cytokines such as IL-12p70 and IL-10. Interestingly, incorporating ethanol-treated tumor cells and TLRs-stimulated DCs during the fusion process promoted fusion efficiency and up-regulated MHC class II molecules on a per fusion basis. Moreover, fusions of ethanol-treated tumor cells and dual TLRs-stimulated DCs (E-tumor/FCs) inhibited the production of multiple immunesuppressive soluble factors including TGF-b1 and up-regulated the production of IL-12p70 and HSP90a. Most importantly, Etumor/ FCs activated T cells capable of producing high levels of IFN-c, resulting in augmented MUC1-specific CTL induction. Collectively, our results illustrate the synergy between ethanol-treated whole tumor cells and dual TLRs-stimulated DCs in inducing augmented CTL responses in vitro by FC preparations. The alternative system is simple and may provide a platform for adoptive immunotherapy.

 

Click To View

Additional Books


  • Plos One : Intravenous Application of a ... (by )
  • Plos One : Influenza Aerosols in Uk Hosp... (by )
  • Plos One : Examination of Marco Activity... (by )
  • Plos One : Proper Indication of Brafv600... (by )
  • Plos One : Application of the Asthma Phe... (by )
  • Plos One : Incidence and Mortality of Ac... (by )
  • Plos One : Circulating Micrornas as Spec... (by )
  • Plos One : Identification and Characteri... (by )
  • Plos One : Tracking the Quality of Care ... (by )
  • Plos One : Expression and Activation of ... (by )
  • Plos One : Viscoelastic Cell Mechanics a... (by )
  • Plos One : Education in Health Research ... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.