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Plos One : Crystal Structures of a Plant Trypsin Inhibitor from Enterolobium Contortisiliquum Ecti and of Its Complex with Bovine Trypsin, Volume 8

By Kobe, Bostjan

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Book Id: WPLBN0003946148
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Reproduction Date: 2015

Title: Plos One : Crystal Structures of a Plant Trypsin Inhibitor from Enterolobium Contortisiliquum Ecti and of Its Complex with Bovine Trypsin, Volume 8  
Author: Kobe, Bostjan
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
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Publisher: Plos

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Kobe, B. (n.d.). Plos One : Crystal Structures of a Plant Trypsin Inhibitor from Enterolobium Contortisiliquum Ecti and of Its Complex with Bovine Trypsin, Volume 8. Retrieved from http://netlibrary.net/


Description
Description : A serine protease inhibitor from Enterolobium contortisiliquum (EcTI) belongs to the Kunitz family of plant inhibitors, common in plant seeds. It was shown that EcTI inhibits the invasion of gastric cancer cells through alterations in integrindependent cell signaling pathway. We determined high-resolution crystal structures of free EcTI (at 1.75 A° ) and complexed with bovine trypsin (at 2 A° ). High quality of the resulting electron density maps and the redundancy of structural information indicated that the sequence of the crystallized isoform contained 176 residues and differed from the one published previously. The structure of the complex confirmed the standard inhibitory mechanism in which the reactive loop of the inhibitor is docked into trypsin active site with the side chains of Arg64 and Ile65 occupying the S1 and S19 pockets, respectively. The overall conformation of the reactive loop undergoes only minor adjustments upon binding to trypsin. Larger deviations are seen in the vicinity of Arg64, driven by the needs to satisfy specificity requirements. A comparison of the EcTI-trypsin complex with the complexes of related Kunitz inhibitors has shown that rigid body rotation of the inhibitors by as much as 15u is required for accurate juxtaposition of the reactive loop with the active site while preserving its conformation. Modeling of the putative complexes of EcTI with several serine proteases and a comparison with equivalent models for other Kunitz inhibitors elucidated the structural basis for the fine differences in their specificity, providing tools that might allow modification of their potency towards the individual enzymes.

 

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