World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Deletion of C7L and K1L Genes Leads to Significantly Decreased Virulence of Recombinant Vaccinia Virus Tiantan, Volume 8

By Wu, Yuntao

Click here to view

Book Id: WPLBN0003946341
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Deletion of C7L and K1L Genes Leads to Significantly Decreased Virulence of Recombinant Vaccinia Virus Tiantan, Volume 8  
Author: Wu, Yuntao
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Wu, Y. (n.d.). Plos One : Deletion of C7L and K1L Genes Leads to Significantly Decreased Virulence of Recombinant Vaccinia Virus Tiantan, Volume 8. Retrieved from http://netlibrary.net/


Description
Description : The vaccinia virus TianTan (VTT) has been modified as an HIV vaccine vector in China and has shown excellent performance in immunogenicity and safety. However, its adverse effects in immunosuppressed individuals warrant the search for a safer vector in the following clinic trails. In this study, we deleted the C7L and K1L genes of VTT and constructed six recombinant vaccinia strains VTT△C7L, VTT△K1L, VTT△C7LK1L, VTKgpe△C7L, VTKgpe△K1L and VTT△C7LK1L-gag. The pathogenicity and immunogenicity of these recombinants were evaluated in mouse and rabbit models. Comparing to parental VTT, VTT△C7L and VTT△K1L showed significantly decreased replication capability in CEF, Vero, BHK-21 and HeLa cell lines. In particular, replication of VTT△C7LK1L decreased more than 10-fold in all four cell lines. The virulence of all these mutants were decreased in BALB/c mouse and rabbit models: VTT△C7LK1L once again showed the greatest attenuation, having resulted in no evident damage in mice and erythema of only 0.4 cm diameter in rabbits, compared to 1.48 cm for VTT. VTKgpe△C7L, VTKgpe△K1L and VTT△C7LK1L-gag elicited as strong cellular and humoral responses against HIV genes as did VTKgpe, while humoral immune response against the vaccinia itself was reduced by 4-8-fold. These data show that deletion of C7L and K1L genes leads to significantly decreased virulence without compromising animal host immunogenicity, and may thus be key to creating a more safe and effective HIV vaccine vector.

 

Click To View

Additional Books


  • Plos One : Inferring Processes from Spat... (by )
  • Plos One : Incidence of and Risk Factors... (by )
  • Plos One : Type 1 Interferons Inhibit My... (by )
  • Plos One : the Amyloid-b-sdr5C1Abad Inte... (by )
  • Plos One : Atlantic Origin of the Arctic... (by )
  • Plos One : Population Genetic Structure ... (by )
  • Plos One : Intra and Interobserver Relia... (by )
  • Plos One : the Different Role of Notch1 ... (by )
  • Plos One : a New Non-human Primate Model... (by )
  • Plos One : the Prolyl Isomerase Pin1 Act... (by )
  • Plos One : Design Optimization of Pin Fi... (by )
  • Plos One : Baseline Monitoring of the We... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.