World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Delivery of Therapeutic Agt Shrna by Peg-bu for Hypertension Therapy, Volume 8

By Pandit, Abhay

Click here to view

Book Id: WPLBN0003946359
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Delivery of Therapeutic Agt Shrna by Peg-bu for Hypertension Therapy, Volume 8  
Author: Pandit, Abhay
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Pandit, A. (n.d.). Plos One : Delivery of Therapeutic Agt Shrna by Peg-bu for Hypertension Therapy, Volume 8. Retrieved from http://netlibrary.net/


Description
Description : Gene silencing by RNA interference (RNAi) is a promising approach for gene therapy. However, up to today, it is still a major challenge to find safe and efficient non-viral vectors. Previously, we reported PEI-Bu, a small molecular weight PEI derivative, as an efficient non-viral carrier. However, like many PEI-based polymers, PEI-Bu was too toxic. In order to reduce cytotoxicity while maintain or even enhance transfecion efficiency, we modified PEI-Bu with poly(ethylene glycol) (PEG) to obtain PEGBu, and used it to delivery a theraputic short hairpin RNA (shRNA) targeting angiotensinogen (AGT) to normal rat liver cells (BRL-3A), which was a key target for the treatment of hypertension. The structure of PEG-Bu was confirmed by proton nuclear magnetic resonance (1H-NMR). Gel permeation chromatography (GPC) showed that the weight average molecular weight (Mw) of PEG-Bu was 5880 Da, with a polydispersity of 1.58. PEG-Bu could condense gene cargo into spherical and uniform nanoparticles with particle size (65–88 nm) and zeta potential (7.3–9.6 mV). Interestingly and importantly, PEG-Bu displayed lower cytotoxicity and enhanced tranfection efficiency than PEI-Bu after PEGylation in both normal cells BRL-3A and tumor cells HeLa. Moreover, PEG-Bu could efficiently delivery AGT shRNA to knockdown the AGT expression. To sum up, PEG-Bu would be a promising non-viral vector for delivering AGT shRNA to BRL-3A cells for hypertension therapy.

 

Click To View

Additional Books


  • Plos One : the Statistical Analysis of M... (by )
  • Plos One : Association Between Myocardia... (by )
  • Plos One : Sfrp5 Modulates Both Wnt and ... (by )
  • Plos One : Gene Expression and Dna-methy... (by )
  • Plos One : Multivariate Modeling of Prot... (by )
  • Plos One : Contribution of Stochastic Pa... (by )
  • Plos One : Study on Citrus Response to H... (by )
  • Plos One : Regulation of Monocyte Adhesi... (by )
  • Plos One : Closing the Gap Between Singl... (by )
  • Plos One : Early Detection of Nsclc with... (by )
  • Plos One : Compromised Mitochondrial Fat... (by )
  • Plos One : Enhancing Communication About... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.