World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Diversity in the Major Polysaccharide Antigen of Acinetobacter Baumanniiassessed by Dna Sequencing, and Development of a Molecular Serotyping Scheme, Volume 8

By Nubel, Ulrich

Click here to view

Book Id: WPLBN0003946957
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Diversity in the Major Polysaccharide Antigen of Acinetobacter Baumanniiassessed by Dna Sequencing, and Development of a Molecular Serotyping Scheme, Volume 8  
Author: Nubel, Ulrich
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Nubel, U. (n.d.). Plos One : Diversity in the Major Polysaccharide Antigen of Acinetobacter Baumanniiassessed by Dna Sequencing, and Development of a Molecular Serotyping Scheme, Volume 8. Retrieved from http://netlibrary.net/


Description
Description : We have sequenced the gene clusters for type strains of the Acinetobacter baumannii serotyping scheme developed in the 1990s, and used the sequences to better understand diversity in surface polysaccharides of the genus. We obtained genome sequences for 27 available serovar type strains, and identified 25 polysaccharide gene cluster sequences. There are structures for 12 of these polysaccharides, and in general the genes present are appropriate to the structure where known. This greatly facilitates interpretation. We also find 53 different glycosyltransferase genes, and for 7 strains can provisionally allocate specific genes to all linkages. We identified primers that will distinguish the 25 sequence forms by PCR or microarray, or alternatively the genes can be used to determine serotype by ‘‘molecular serology’’. We applied the latter to 190 Acinetobacter genome-derived gene-clusters, and found 76 that have one of the 25 gene-cluster forms. We also found novel gene clusters and added 52 new gene-cluster sequence forms with different wzy genes and different gene contents. Altogether, the strains that have one of the original 25 sequence forms include 98 A. baumannii (24 from our strains) and 5 A. nosocomialis (3 from our strains), whereas 32 genomes from 12 species other than A. baumannii or A. nosocomialis, all have new sequence forms. One of the 25 serovar type sequences is found to be in European clone I (EC I), 2 are in EC II but none in EC III. The public genome strains add an additional 52 new sequence forms, and also bring the number found in EC I to 5, in EC II to 9 and in EC III to 2.

 

Click To View

Additional Books


  • Plos One : Why Even More Clinical Resear... (by )
  • Plos One : Cyclic Occurrence of Fire and... (by )
  • Plos One : Effect of Parenteral Selenium... (by )
  • Plos One : the Inhibition of Autophagy S... (by )
  • Plos One : Does Trophic Status Enhance o... (by )
  • Plos One : Automatic Quantitative Mri Te... (by )
  • Plos One : Factors Controlling Soil Micr... (by )
  • Plos One : Cognitive Decline in Older Pe... (by )
  • Plos One : Age-specific Average Head Tem... (by )
  • Plos One : Differential Requirement for ... (by )
  • Plos One : Quantification of the Early S... (by )
  • Plos One : Sight of a Predator Induces a... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.