World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Glycogen Synthase Kinase-3 Inhibition Enhances Translation of Pluripotency-associated Transcription Factors to Contribute to Maintenance of Mouse Embryonic Stem Cell Self-renewal, Volume 8

By Cooney, John, Austin

Click here to view

Book Id: WPLBN0003949261
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Glycogen Synthase Kinase-3 Inhibition Enhances Translation of Pluripotency-associated Transcription Factors to Contribute to Maintenance of Mouse Embryonic Stem Cell Self-renewal, Volume 8  
Author: Cooney, John, Austin
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Cooney, J. A. (n.d.). Plos One : Glycogen Synthase Kinase-3 Inhibition Enhances Translation of Pluripotency-associated Transcription Factors to Contribute to Maintenance of Mouse Embryonic Stem Cell Self-renewal, Volume 8. Retrieved from http://netlibrary.net/


Description
Description : Maintenance of embryonic stem cell (ESC) self-renewal and pluripotency are controlled by extrinsic factors, molecular signaling pathways and transcriptional regulators. While many of the key players have been studied in depth, how the molecular signals interact with transcription factors of the pluripotency network to regulate their action remains less well understood. Inhibition of glycogen synthase kinase 3 (Gsk-3) has been implicated in the maintenance of mouse ESC pluripotency, although there is contradictory data on its role, with enhancement of cell survival and metabolism, stabilisation of c-Myc and activation of Wnt signalling proposed as potential mechanisms. We have discovered that suppression of Gsk-3 activity leads to enhanced protein levels of key transcriptional regulators of the pluripotency network, notably Nanog, Tbx3 and c-Myc. Protein stability was unchanged following Gsk-3 inhibition, although interestingly, Nanog and Tbx3 proteins were found to have half-lives of 1–3 h, while that of Oct4 protein was longer, at 6 h. We demonstrate that the effects on protein levels seen following inhibition of Gsk-3 are due to both enhanced de novo synthesis of Nanog protein and increases in the proportion of Nanog and Tbx3 RNAs bound to polysomes, findings consistent with Gsk-3 regulating translation of these factors. These effects were not due to changes in regulators of general translation initiation machinery nor mediated via the 59 or 39 UTR sequences of Nanog alone. The data we present provide both new conceptual insight into the mechanisms regulated by Gsk-3 that may contribute to ESC self-renewal and, importantly, establish control of protein translation as an additional mechanism involved in modulation of ESC pluripotency.

 

Click To View

Additional Books


  • Plos One : Skin Lesions in European Hibe... (by )
  • Plos One : Prostaglandins, Masculinizati... (by )
  • Plos One : Inhibition of Ube2D3 Expressi... (by )
  • Plos One : Forkhead Box Protein A2 Foxa2... (by )
  • Plos One : Complete Chloroplast Genome S... (by )
  • Plos One : Angiotensin-1–7 in Paraventri... (by )
  • Plos One : Factors Associated with Esoph... (by )
  • Plos One : Re-evaluation of Sarcolemma I... (by )
  • Plos One : Nanoceria ; a Rare-earth Nano... (by )
  • Plos One : Sulforaphane Induces Cell Cyc... (by )
  • Plos One : Functional Analysis of Casein... (by )
  • Plos One : Bladder Pain Syndrome, Volume... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.