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Plos One : Mberfp, an Improved Large Stokes Shift Red Fluorescent Protein, Volume 8

By Jones, D., Dafydd

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Book Id: WPLBN0003951796
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos One : Mberfp, an Improved Large Stokes Shift Red Fluorescent Protein, Volume 8  
Author: Jones, D., Dafydd
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

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Jones, D. D. (n.d.). Plos One : Mberfp, an Improved Large Stokes Shift Red Fluorescent Protein, Volume 8. Retrieved from http://netlibrary.net/


Description
Description : Herein, we describe the generation of a monomeric large Stokes shift (LSS) red fluorescent protein, mBeRFP, with excitation and emission peaks at 446 and 615 nm, respectively. Compared with two previously reported LSS-RFPs (mKeima and LSSmKate2), mBeRFP is approximately three times brighter. In addition, mBeRFP is characterized by improved photostability, rapid maturation, an extended lifetime, and a monomeric nature. Additionally, mBeRFP can be paired with the Alexa 647 dye as a FRET donor to detect caspase 3 activity. This FRET pair has an extremely dynamic range and a large Fo¨ rster radius (approximately 6.5 nm). To demonstrate the applicability of mBeRFP for imaging in living cells, we performed dual-color imaging of mBeRFP and CFP simultaneously excited by a single excitation source, and we demonstrated that these fluorescent proteins allow the clear visualization of the dynamics of Bax during cancer cell apoptosis. Thus, mBeRFP appears to be particularly useful for cellular imaging applications.

 

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