World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos Biology : Snw1 is a Critical Regulator of Spatial Bmp Activity, Neural Plate Border Formation, and Neural Crest Specification in Vertebrate Embryos, Volume 9

By L. Stemple, Derek

Click here to view

Book Id: WPLBN0003952305
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos Biology : Snw1 is a Critical Regulator of Spatial Bmp Activity, Neural Plate Border Formation, and Neural Crest Specification in Vertebrate Embryos, Volume 9  
Author: L. Stemple, Derek
Volume: Volume 9
Language: English
Subject: Journals, Science, Biology
Collections: Periodicals: Journal and Magazine Collection, PLoS Biology
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Stemple, D. L. (n.d.). Plos Biology : Snw1 is a Critical Regulator of Spatial Bmp Activity, Neural Plate Border Formation, and Neural Crest Specification in Vertebrate Embryos, Volume 9. Retrieved from http://netlibrary.net/


Description
Description : Bone morphogenetic protein (BMP) gradients provide positional information to direct cell fate specification, such as patterning of the vertebrate ectoderm into neural, neural crest, and epidermal tissues, with precise borders segregating these domains. However, little is known about how BMP activity is regulated spatially and temporally during vertebrate development to contribute to embryonic patterning, and more specifically to neural crest formation. Through a large-scale in vivo functional screen in Xenopus for neural crest fate, we identified an essential regulator of BMP activity, SNW1. SNW1 is a nuclear protein known to regulate gene expression. Using antisense morpholinos to deplete SNW1 protein in both Xenopus and zebrafish embryos, we demonstrate that dorsally expressed SNW1 is required for neural crest specification, and this is independent of mesoderm formation and gastrulation morphogenetic movements. By exploiting a combination of immunostaining for phosphorylated Smad1 in Xenopus embryos and a BMP-dependent reporter transgenic zebrafish line, we show that SNW1 regulates a specific domain of BMP activity in the dorsal ectoderm at the neural plate border at postgastrula stages. We use double in situ hybridizations and immunofluorescence to show how this domain of BMP activity is spatially positioned relative to the neural crest domain and that of SNW1 expression. Further in vivo and in vitro assays using cell culture and tissue explants allow us to conclude that SNW1 acts upstream of the BMP receptors. Finally, we show that the requirement of SNW1 for neural crest specification is through its ability to regulate BMP activity, as we demonstrate that targeted overexpression of BMP to the neural plate border is sufficient to restore neural crest formation in Xenopus SNW1 morphants. We conclude that through its ability to regulate a specific domain of BMP activity in the vertebrate embryo, SNW1 is a critical regulator of neural plate border formation and thus neural crest specification.

 

Click To View

Additional Books


  • Plos Biology : Molecular Composition and... (by )
  • Plos Biology : Ltp Promotes a Selective ... (by )
  • Plos Biology : Global Gradients in Verte... (by )
  • Plos Biology : Are We Really Getting Con... (by )
  • Plos Biology : Local CA2+ Entry Via Orai... (by )
  • Plos Biology : Neuronal Expression of Gl... (by )
  • Plos Biology : Mind the Gap!, Volume 7 (by )
  • Plos Biology : Hormonal Signal Amplifica... (by )
  • Plos Biology : Stepwise Development of M... (by )
  • Plos Biology : Evolution Acts on Enhance... (by )
  • Plos Biology : Being Positive About Sele... (by )
  • Plos Biology : Traversing the Highwire f... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.