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Plos One : the Epithelial Sodium Channel Enac Establishes a Trafficking Vesicle Pool Responsible for Its Regulation, Volume 7

By Sands, Jeff, M

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Book Id: WPLBN0003961011
Format Type: PDF eBook :
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Reproduction Date: 2015

Title: Plos One : the Epithelial Sodium Channel Enac Establishes a Trafficking Vesicle Pool Responsible for Its Regulation, Volume 7  
Author: Sands, Jeff, M
Volume: Volume 7
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection
Historic
Publication Date:
Publisher: Plos

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Sands, J. M. (n.d.). Plos One : the Epithelial Sodium Channel Enac Establishes a Trafficking Vesicle Pool Responsible for Its Regulation, Volume 7. Retrieved from http://netlibrary.net/


Description
Description : The epithelial sodium channel (ENaC) is the rate-limiting step for sodium reabsorption across tight epithelia. Cyclic-AMP (cAMP) stimulation promotes ENaC trafficking to the apical surface to increase channel number and transcellular Na+ transport. Removal of corticosteroid supplementation in a cultured cortical collecting duct cell line reduced ENaC expression. Concurrently, the number of vesicles trafficked in response to cAMP stimulation, as measured by a change in membrane capacitance, also decreased. Stimulation with aldosterone restored both the basal and cAMP-stimulated ENaC activity and increased the number of exocytosed vesicles. Knocking down ENaC directly decreased both the cAMPstimulated short-circuit current and capacitance response in the presence of aldosterone. However, constitutive apical recycling of the Immunoglobulin A receptor was unaffected by alterations in ENaC expression or trafficking. Fischer Rat Thyroid cells, transfected with a,b,c-mENaC had a significantly greater membrane capacitance response to cAMP stimulation compared to non-ENaC controls. Finally, immunofluorescent labeling and quantitation revealed a smaller number of vesicles in cells where ENaC expression was reduced. These findings indicate that ENaC is not a passive passenger in regulated epithelial vesicle trafficking, but plays a role in establishing and maintaining the pool of vesicles that respond to cAMP stimulation

 

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