World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Ovarian Cancer Stem Cells Are Enriched in Side Population and Aldehyde Dehydrogenase Bright Overlapping Population, Volume 8

By Hawkins, Shannon, M.

Click here to view

Book Id: WPLBN0003964293
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Ovarian Cancer Stem Cells Are Enriched in Side Population and Aldehyde Dehydrogenase Bright Overlapping Population, Volume 8  
Author: Hawkins, Shannon, M.
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Hawkins, S. M. (n.d.). Plos One : Ovarian Cancer Stem Cells Are Enriched in Side Population and Aldehyde Dehydrogenase Bright Overlapping Population, Volume 8. Retrieved from http://netlibrary.net/


Description
Description : Cancer stem-like cells (CSCs)/cancer-initiaiting cells (CICs) are defined as a small population of cancer cells that have selfrenewal capacity, differentiation potential and high tumor-initiating ability. CSCs/CICs of ovarian cancer have been isolated by side population (SP) analysis, ALDEFLUOR assay and using cell surface markers. However, these approaches are not definitive markers for CSCs/CICs, and it is necessary to refine recent methods for identifying more highly purified CSCs/CICs. In this study, we analyzed SP cells and aldehyde dehydrogenese bright (ALDHBr) cells from ovarian cancer cells. Both SP cells and ALDHBr cells exhibited higher tumor-initiating ability and higher expression level of a stem cell marker, sex determining region Y-box 2 (SOX2), than those of main population (MP) cells and ALDHLow cells, respectively. We analyzed an SP and ALDHBr overlapping population (SP/ALDHBr), and the SP/ALDHBr population exhibited higher tumor-initiating ability than that of SP cells or ALDHBr cells, enabling initiation of tumor with as few as 102 cells. Furthermore, SP/ADLHBr population showed higher sphere-forming ability, cisplatin resistance, adipocyte differentiation ability and expression of SOX2 than those of SP/ALDHLow, MP/ALDHBr and MP/ALDHLow cells. Gene knockdown of SOX2 suppressed the tumor-initiation of ovarian cancer cells. An SP/ALDHBr population was detected in several gynecological cancer cells with ratios of 0.1% for HEC—1 endometrioid adenocarcinoma cells to 1% for MCAS ovary mucinous adenocarcinoma cells. Taken together, use of the SP and ALDHBr overlapping population is a promising approach to isolate highly purified CSCs/CICs and SOX2 might be a novel functional marker for ovarian CSCs/CICs.

 

Click To View

Additional Books


  • Plos One : Enhanced Persistency of Resti... (by )
  • Plos One : Aaerf1 Positively Regulates t... (by )
  • Plos One : Inhibition of Spontaneous Rec... (by )
  • Plos One : Repositioning Antimicrobial A... (by )
  • Plos One : Close to You ; Embodied Simul... (by )
  • Plos One : the Role of Nucleus Accumbens... (by )
  • Plos One : Transcriptional and Metabolic... (by )
  • Plos One : Pet Analysis of Dopaminergic ... (by )
  • Plos One : Spontaneous Reperfusion After... (by )
  • Plos One : Analyzing the Modification of... (by )
  • Plos One : Reduced T2* Values in Soleus ... (by )
  • Plos One : Identification of Human Monoc... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.