World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

Plos One : Recombinant Mammaglobin a Adenovirus-infected Dendritic Cells Induce Mammaglobin A-specific Cd8+ Cytotoxic T Lymphocytes Against Breast Cancer Cells in Vitro, Volume 8

By Karagiannis, Sophia, N.

Click here to view

Book Id: WPLBN0003965754
Format Type: PDF eBook :
File Size:
Reproduction Date: 2015

Title: Plos One : Recombinant Mammaglobin a Adenovirus-infected Dendritic Cells Induce Mammaglobin A-specific Cd8+ Cytotoxic T Lymphocytes Against Breast Cancer Cells in Vitro, Volume 8  
Author: Karagiannis, Sophia, N.
Volume: Volume 8
Language: English
Subject: Journals, Science, Medical Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary)
Historic
Publication Date:
Publisher: Plos

Citation

APA MLA Chicago

Karagiannis, S. N. (n.d.). Plos One : Recombinant Mammaglobin a Adenovirus-infected Dendritic Cells Induce Mammaglobin A-specific Cd8+ Cytotoxic T Lymphocytes Against Breast Cancer Cells in Vitro, Volume 8. Retrieved from http://netlibrary.net/


Description
Description : Mammaglobin A (MGBA) is a novel breast cancer-associated antigen almost exclusively over-expressed in primary and metastatic human breast cancers, making it a potential therapeutic target for breast cancer. The development of dendritic cell (DC)-induced tumor antigen specific CD8+ cytotoxic T lymphocytes (CTLs) may hold promise in cancer immunotherapy. In this study we constructed recombinant replication-defective adenoviral (Ad) vectors encoding MGBA and evaluated their ability to trigger anti-tumor immunity in vitro. DCs were isolated from the human peripheral blood monocyte cells (PBMCs) of two HLA-A33+ healthy female volunteers, and infected with adenovirus carrying MGBA cDNA (Ad-MGBA). After that, the Ad-MGBA-infected DCs were used to stimulate CD8+ CTLs in vitro and the latter was used for co-culture with breast cancer cell lines. The data revealed that infection with Ad-MGBA improved DC maturation and up-regulated the expression of costimulatory molecules and the secretion of interleukin-12 (IL-12), but down-regulated interleukin-10 (IL-10) secretion from DCs. Ad-MGBA-infected DC-stimulated CD8+CTLs displayed the highest cytotoxicity towards HLA-A33+/MGBA+ breast cancer MDA-MB-415 cells compared with other CD8+CTL populations, and compared with the cytotoxicity towards HLAA33 2/MGBA+ breast cancer HBL-100 cells and HLA-A332/MGBA2 breast cancer MDA-MB 231 cells. In addition, Ad-MGBAinfected DC-stimulated CD8+ CTLs showed a high level of IFNc secretion when stimulated with HLA-A33+/MGBA+ breast cancer MDA-MB-415 cells, but not when stimulated with HLA-A332/MGBA+ HBL-100 and HLA-A332/MGBA2MDA-MB-231 cells. In addition, killing of CD8+CTLs against breast cancer was in a major histocompability complex (MHC)-limited pattern. Finally, the data also determined the importance of TNF-a in activating DCs and T cells. These data together suggest that MGBA recombinant adenovirus-infected DCs could induce specific anti-tumor immunity against MGBA+ breast cancers, which could provide a novel strategy in the immunotherapy of breast cancer.

 

Click To View

Additional Books


  • Plos One : Diversity in Protein Profiles... (by )
  • Plos One : Superficial Nephrons in Balb,... (by )
  • Plos One : Multi-parametric Clustering f... (by )
  • Plos One : Passive Immunization with Hyp... (by )
  • Plos One : Distinct Roles of Molecular C... (by )
  • Plos One : the Sequence Structures of Hu... (by )
  • Plos One : Mental Quality of Life is Rel... (by )
  • Plos One : Novel Mecp2 Isoform-specific ... (by )
  • Plos One : a Test for Pre-adapted Phenot... (by )
  • Plos One : Love and Suicide ; the Struct... (by )
  • Plos One : Application of Intra-oral Den... (by )
  • Plos One : External Validation of a Nomo... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from World Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.